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Business and Human Rights | 2013 Deitelhoff, Nicole

E2F transcription factor-1 modulates expression of glutamine metabolic genes in mouse embryonic fibroblasts and uterine sarcoma cells

Pellets were thawed on ice and 750 mL of FA buffer containing protease inhibitors (Roche Cat#11697498001 Complete Protease Inhibitor Cocktail Tablet, 125 L 100 mM PMSF, and 25 L 1 M DTT per 25 mL FA buffer) was added, and samples were then transferred to a 2 mL KONTES dounce (Kimble Chase, Vineland, NJ)

In summary, the exogenous SL effectively compensated for drought-induced functional inhibition, a process closely associated with the up-regulation of key defense pathways at the transcriptional level, such as flavonoids, triterpenoids, and amino acid metabolism