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Total RNA isolation and mRNA sequencing sample preparation The total RNA isolation and sequencing library preparation from IR-induced senescent and proliferating IMR90 cells was performed as follows: total RNA was isolated using Qiazol Lysis Reagent (Qiagen) and purified with the miRNeasy kit (Qiagen)

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their healing effects on gastrointestinal tract lesions (Seiwerth et al., 2018)

TFA Removal: Processed via acetate exchange to remove inflammatory trifluoroacetic acid salts, ensuring high viability in sensitive cell culture applications