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Nonetheless, because an increase in oxidant production does not necessarily define a pro-oxidant condition, measures of increased oxidant production alone are not definitive markers of oxidative stress

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Subcellular localization predictions were performed using WoLF-PSORT ( and PSORTb ( 2.7 Co-immunoprecipitation Based on the sequences of identified interacting proteins PEX3 and TRAP1, primers containing BamHI and XhoI restriction sites were designed to amplify these genes by PCR (Table 1)

Standard polymerase chain reaction (PCR) was used to amplify the prkn and Sod2 loci