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No human safety data : The complete absence of human clinical trials means the safety profile, side effects, optimal dosing, and pharmacokinetics in humans are entirely unknown Animal-to-human translation is uncertain : Metabolic differences between mice and humans mean that the magnitude of effects (e.g., weight loss, grip strength improvement) may not translate

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Foam means air has been incorporated into the solution, which introduces oxidation and makes accurate volume measurements impossible until the foam settles

The nuclear protein fraction was extracted, and the relative Dnmt and 5-mC hydroxylase Tet activities were calculated based on the ratio of the treatment group to the control group after the normalization to the amount of protein