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Role of the Hypothalamic-pituitary-adrenal axis in health and disease

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The cytosolic and nuclear proteins were isolated as described in the nucleoprotein extraction kit (Beyotime Biotechnology, Nanjing, Jiangsu, China)

Compared to UBE2m RK, fatty acid metabolism and mitochondrial respiration were inhibited after re-expression of UBE2m WT, and the suppressed OCR was rescued significantly after treatment with MLN4924 (Figs