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The ionization state of these side chains affects the physical and chemical properties of proteins and is important for their interactions with other proteins, substrates or ligands, and other macromolecules as well as for their physiological functions

doi:10.1016/j.clnu.2019.03.020

Certain drugs (such steroids), smoking, or chronic diseases like leukemia can also cause it

2.6 Extraction and measurement of membrane lipids Half-gram intact broccolini tissue samples were soaked in a 5 mL propan-2-ol solution containing 0.01% butylated hydroxytoluene (BHT) in a water bath at 75 C for 10 min