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A comprehensive analysis of REE in single-Trp proteins revealed that the effects can be found in proteins possessing fluorescence emission spectra with max ranging from 320 to 340 nm, whereas in proteins and peptides with fluorescence spectra above 340 nm, REE are not observable because of the high flexibility of these proteins and the access of tryptophan residues to the rapidly relaxing solvent

No significant differences between treatment groups were observed using the Chao1 richness estimate 42 , which accounts for ASV rareness in the richness measure (Supplementary Fig

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