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The occluded aqueous cavity housing the Na + -binding site is unusual, and we hypothesise that Na + binding and ordering of solvent within this cavity may stabilise the N-terminal domain and contribute to transporter gating

After blocking the membrane in 5% skim milk, primary antibody was incubated over night at 4 C

Minor side effects can include: A bit of tenderness, puffiness, or pink discoloration right around the puncture A brief warm or flushed sensation Mild headache or nausea Reach out to a medical professional right away if you notice: Redness that keeps spreading, heat, or discharge near the site (possible infection) Hives, facial swelling, or difficulty breathing (possible allergic reaction) Persistent numbness or tingling that spreads beyond the site Fever or chills within 48 hours of injection Safe Needle Disposal and Storage A proper FDA-cleared sharps bin is ideal