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Nanosci Master Progr 109:120 Toh SY, Citartan M, Gopinath SCB, Tang T-H (2015) Aptamers as a replacement for antibodies in enzyme-linked immunosorbent assay

Tissue sections or fixed endothelial cells were processed for immunolabeling by permeabilization (0.4% Triton X-100 in PBS), blocking (4% horse serum, 2% BSA, 0.2% Triton X-100 in PBS) and incubation with first antibody (1% HS, 0.05% Triton X-100 in PBS)

NASH severity was monitored by histology and serum liver chemistry analysis

This process is similar to the mobilization and utilization of axunge by the body during exercise or starvation, transforming axunge from a stored state to a state available for energy use