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New strategies for the development of antifungal drugs have gradually become a research hotspot, including the targeting of novel biological pathways, the combination of antifungal drugs, the development of fungal vaccines, the creation of innovative therapeutics (such as small-molecule peptides and nanoparticles), the extraction of active ingredients from traditional Chinese medicine, and the utilization of artificial intelligence in drug design ( Figure 1 ) (Wall and Lopez-Ribot, 2020

Obviously, the overexpression by multicopy plasmid increased the transcription level by increasing its copy number

The answer depends on the biological pathways researchers want to investigate

Silencing of GAS5 alleviates glaucoma in rat models by reducing retinal ganglion cell apoptosis