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After denaturation at 95C for 10 min, the mixture underwent PCR for 40 cycles at 95C for 60 s, annealing temperature for 60 s, and elongation at 72C for 60 s

Transwell assay Matrigel (Shanghai Haoyang Biotechnology, 356234) was thawed at 80 C and equilibrated at 4 C overnight

Activation of the NLRP3 inflammasome leads to the cleavage of procaspase-1 into its active form, which in turn processes proinflammatory cytokines such as IL-1 and IL-18 and drives pyroptotic cell death via Gasdermin D activation

Nanoparticles therapeutic strategies for the treatment human diseases The above-mentioned small molecule ferroptosis regulators have demonstrated potential in preclinical practice for treating various diseases