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linear flow rate 250 cm/h Chemical stability stable in 0.1 M acetate pH 4.0, 0.1 M NaOH, 70 % ethanol or 6 M guanidine hydrochloride for two hours at room temperature, not autoclavable * Tested with human Glutathione S-transferase (GST) Recommended flow rates With a bed volume of 1 ml (10 ml) and an inner diameter of 6.6 mm (16 mm) the flow rates for sample loading should be 0.2 1.0 ml/min (0.5 - 5.0 ml/min) and 1.0 ml/min (5.0 ml/min) for washing/elution

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First, LCA is typically one of the most abundant bile acids in the human colon, and in our assays, it promoted biofilm formation by EcN at concentrations within the ranges reported for human feces and cecal contents

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