This is the same purging mechanism occurring in different locations

The DDA / DSIP System District Development Authorities (DDAs) were established by Parliament in 2014 with the aim to improve local service infrastructure and delivery.1 DDAs are responsible for the spending of District Service Improvement Program (DSIP) and District Infrastructure Program (DIP) funds.2 There are also Service Improvement Program grants that are paid to Provincial and Local Level governments as shown in this diagram: For 2023, each District Development Authority has been allocated a total of K20 million in DSIP and DIP funding in the National Budget.3 DSIP and DIP funds are distributed to the DDAs by the Department of Finance.4 The DSIP funds are to be used by the DDA as follows5: Infrastructure support services (30% / K3 million) Health support services (20% / K2 million) Education support services (20% / K2 million) Law and Justice services (10% / K1 million) Economic support services (10% / K1 million) Administration (10% / K1 million) Each DDA is governed by a Board comprising all the local-level government presidents in the District

The primary antibodies employed were: P53 (Proteintech, 60283-2-Ig), pSer46-P53 (CST, 2521), FOXO4 (Proteintech, 21535-1-AP), P21 (CST, 2947), P16 (CST, 18769), BAX (CST, 2772), BCL2 (CST, 3498), Ki-67 (Proteintech, 84192-4-RR), Lamin B (CST, 13435), Cleaved Caspase 3 (CST, 9664), -H2AX (Abcam, ab81299), and GAPDH (Abcam, ab8245) as an internal reference for normalizing protein expression levels
The first approach (constant concentration, variable volume) simplifies reconstitution but requires precise measurement of small volume changes