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The mis-regulated genes found in the Mettl9 CatD lines represent a much smaller proportion of those previously found in the Mettl9 KO and also smaller than the dTAG V -1-treated Mettl9 Deg lines at the same developmental stage (DIV5) (Fig

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For this, 50 L of beads (Pierce) were blocked with free D-Biotin (Novabiochem) (3 mg/mL prepared in alkaline water (pH 10.5)) and rocked for 1 h at RT, followed by 5 washes with 1 PBS and incubation with the cell lysates (50 L) for 30 min at 4 C with rocking to remove non-specific binding of proteins to the bead complex