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The c-MET receptor tyrosine kinase is overexpressed in H69 cells and is inducible by exogenous HGF, resulting in induction of tyrosine phosphorylation at the major autophosphorylation sites pY1230/1234/1235 in the catalytic kinase domain, and also the pY1003 site in the juxtamembrane domain of c-MET (Figure 1)

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After treatment, cells were immediately analyzed for apoptosis and the cell cycle using a BD FACSCelesta Cell Analyzer with an Annexin V-FITC/PI Apoptosis Detection Kit and PI/RNase Staining Buffer (BD Biosciences, Franklin Lakes, NJ, USA), respectively, according to the manufacturers instructions