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Mediation of androgen metabolism by an optimal concentration of nicotine and serial concentrations of glutathione in cultured gingival and periosteal fibroblasts Incubations were performed with 6 cell-lines of fibroblasts in Eagle's MEM, using 14C-testosterone/14C-4-androstenedione as substrates with an effective concentration of nicotine established previously (N 250 g/ml), in the presence or absence of serial concentrations of glutathione (G, 14 g/ml)

After affinity selection of the fusion protein on the chitin matrix, the intein undergoes specific cleavage by thiol reagents or changes in pH and temperature, thereby releasing the target protein from the chitin-bound tag

Kalarikkal SP, Kumar MN, Rajendran S, Bethi CMS, Ravilla J, Narayanan J, et al