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For SDS-gel electrophoresis proteins were isolated from four to eight cervical spinal cord samples (p40) per genotype using Cell lysis buffer (#9803S, Cell Signaling Technology, Danvers, Massachusetts, USA) supplemented with protease inhibitor (#11697498001, Merck, Darmstadt, Germany)

K.DuX.WangY.LiD.et al

treatment is dependent on personal preference and experience of the treating doctor

Third, adaptive clinical trial designs involving larger patient cohorts and multi-center validation will be crucial to demonstrate efficacy beyond preclinical promise