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Yurgelun-Todd, An exploratory proton MRS examination of gamma-aminobutyric acid, glutamate, and glutamine and their relationship to affective aspects of chronic pain, Neurosci

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Fermentation products (5 mL) were added to 45 mL of sterile normal saline, mixed well, gradient-diluted 10 2 , 10 3 , 10 4 , 10 5 , and 10 6 times with sterile normal saline, and spread on the MRS agar media, which were placed at 37C for 48 h under anaerobic conditions: N 2 (85%), CO 2 (5%), and H 2 (10%) (Luo et al., After the isolates were cultured, single colonies were selected according to colony shape, size, and color