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Furthermore, additional receptors, such as TLR3, TLR4, and IFNAR1, play roles in initiating necroptosis, involving adapter molecules such as TIR domain-containing adapter molecule 1 (TICAM1, also known as TRIF) or RIHM-containing proteins Z-DNA binding protein 1 (ZBP1) [125, 126]

Isolation of PBMCs was performed with Ficoll-Paque density gradient (GE Healthcare)

Elevated levels have also been detected in patients with lupus, rosacea, and some forms of inflammatory bowel disease