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Description

In our experiments, the truncation of the LRRK2 protein through CRISPR/Cas9 gene editing did not produce any detectable detrimental results and provides an alternative method for decreasing kinase activity in a more targeted manner

After blocking, plates were washed four times and 30 l/well of 2 g/ml monomeric His-tagged gH/gL diluted in blocking buffer was added to the plate and incubated for 1 hr