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Importantly, glutathione deficiency was more pronounced in dogs that did not survive to discharge from the hospital, compared to survivors

ROS exhibit a dual role: at low and tightly controlled concentrations, they act as central signalers of growth, development, and stress adaptation

Mf were cleaned and filtered in RPMI 1640 culture media (Gibco, Thermo Fisher Scientific) using PD-10 desalting columns (GE Healthcare) supplemented with 2 mM L-glutamine, 25 mM Hepes, 100 U/mL penicillinstreptomycin (Thermo Fisher Scientific) and 2.5 mg/mL amphotericin B (Thermo Fisher Scientific) at 37 C

doi: 10.1097/WCO.0000000000000730 39