glutathione nmr cosy 1D 1 H spectra recorded in H 2 O at 275K and pH 7.0 for 1H NMR characterization of complexation

In oxidative stressoriented in vitro models, such as primary porcine theca cells, a broader doseresponse evaluation revealed that EV treatment (30120 g/mL for 24 h) reduced reactive oxygen species accumulation, limited apoptosis, and enhanced steroidogenic activity, underscoring the importance of aligning EV dose and duration with the specific stress paradigm and cellular target (Yuan et al., 2022)

doi: 10.3390/cells9040828 63 YangJPengSZhangK

coli plasmid (Supplementary Table S1) were independently introduced in Synechocystis by transformation (Supplementary Table S2 and Figures 1A,C,E) to verify that the Km r or Sm r /Sp r marker genes had properly replaced sll1545 or slr0236 in the polyplod Synechocystis chromosome (it occurs, at about ten copies per cell r or Sm r /Sp r ) chromosome copies was complete (the gene is dispensable to cell growth) or not (the gene is essential to cell viability)

PCR was performed in 20 l reaction mixture containing 5 ng of the obtained cDNA (or 50 ng of human genomic DNA as a control), 1x Phusion HF buffer, 0.2 mM dNTP mix, 0.5 M of each primer and 0.4 U Phusion DNA polymerase (F530S, Thermo Scientific)