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Biochim Biophys Acta, 2013

The solutions were combined under continuous agitation at ambient temperature, followed by hydrothermal treatment at 95 C for 5 h in a Teflon-lined autoclave

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Jesse Rinehart, an associate professor of cellular and molecular physiology at the Yale School of Medicine and co-senior author on the study, called the breakthrough a profound piece of whole genome engineering based on over 1,000 precise edits at a scale an order of magnitude greater than any engineering feat we have previously done. This is an exciting new platform technology that opens up an array of applications for biotechnology both in the academic realm and in the commercial sector, Rinehart said