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Pharmaceutical-grade peptides used in clinical research are lyophilised, shipped on dry ice, stored at 20C until reconstitution, and used within 35 days under sterile protocol

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Inflammatory mediators, including cytokines, chemokines, and reactive oxygen species (ROS), are released during I/R and travel to the brain, where they can directly damage the BBB and promote neuroinflammation (Song et al., 2020)

Ultra-pure water was received from water purification Milli-Q system (Millipore Corporation, USA)