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6.4 Inhibition of AGEs/RAGE pathway The accumulation of AGEs is a prominent characteristic of diabetes and its complications, particularly DKD

The new supernatant (cytosolic fraction) was directly prepared for SDS-PAGE, and the pellet (membrane fraction) was gently washed with hypo-osmotic buffer and resuspended in GST-lysis buffer supplemented with 0.5% SDS and protease inhibitors

Targeting pathways such as AURKB, PI3KAKT, JNK, and FAKYAP has been shown to prevent survival under therapeutic stress (52, 57, 62, 63)

Case reports and small trials show noticeable regrowth and nail thickening once levels are corrected