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This enzyme forms the hemithiolacetal into S-D- lactoylglutathione, then Gly II converts the molecule further to D-lactate

Assessment of the Withdrawal Threshold for the Hind Paw Mice in the four groups were tested for pain sensitivity using von Frey monofilaments (20PC Aesthe, Muromachi Kikai Ltd., Tokyo, Japan) and a hot plate (Variomag, Langenselbold, Germany)