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Rats were survived for 5 additional days to allow for maximal retrograde transport of the tracer FG to the perikaya of spinal motor neurons, and sacrificed under deep anesthesia by transcardial perfusion with saline and subsequently buffered 4% paraformaldehyde

Manufactured in USA facilities using pharmaceutical-grade synthesis protocols and verified through independent laboratory analysis

Overexpression of HOTTIP increased cell proliferation, whereas its knockdown decreased it

First, field researchincluding street observations, interviews, and photo documentationdocuments various grassroots practices, including composting, recultivating idle farmland, and operating informal street markets