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The system was controlled using MassHunter 12 software (Agilent, Palo Alto, CA, USA)

We will have Dan back again very soon!" "Keeping S.G IV on speed dial

HCT116 Flp-in cells (seeded in 10 cm plates, 30% confluency) were co-transfected with 9 g of donor plasmid and 3 g of sgRNA-Cas9 plasmid, and 24 l Turbofect (Thermo Scientific, R0533) in 2 ml Opti-MEM (Thermo Scientific, 11058-021)

doi: 10.3389/fpls.2022.802274 Received 26 October 2021 Accepted 14 March 2022 Published 25 April 2022 Volume 13 - 2022 Edited by Dirceu Mattos Jr, Instituto Agronmico de Campinas (IAC), Brazil Reviewed by Franz Hippler, Yara International, Brazil