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Yang X, Lee S, So JH, Dharmasiri S, Dharmasiri N, Ge L, et al

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Seven days after microinjection, the rats were again anesthetized with ketamine and xylazine, and perfused through the left cardiac ventricle with cold, oxygen-enriched, Ca 2+ -free Tyrodes buffer (40 ml at 4C) and ice-cold paraformaldehyde (200 ml, 4% (w/v) in 0.1 m sodium phosphate buffer, pH 7.3) for 15 min at a pressure of 50 mmHg with a perfusion pump (L/S peristaltic tubing pump, Masterflex)

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