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Igf1 CART-KO also presented less staining of the cartilage degrading enzymes MMP13 and ADAMTS5 compared to control mice, upon DMM (Supplementary Fig

At the end of incubation, 20 L of MTS reagent was added to each well and incubated for another two hours, then analyzed with a microplate reader using a test wavelength of 490 nm within 10 min