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15 N and 13 C incorporation assays In the 15 N incorporation assay, cells were inoculated into 5 mL KDC medium in 25 test tubes, which were stoppered with butyl-rubber, and the gas phase was replaced with a mixture of 15 N 2 :O 2 (82:18) (Shoko Science, Kanagawa, Japan) for the 15 N 2 sample or air for the 14 N 2 sample

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Our findings are consistent with previous studies, suggesting that butyrate may inhibit the LPS-induced activation of the TLR4/NF-B pathway, thereby exerting anti-inflammatory effects [54]