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Sci Rep 7:4537 Ribas A, Wolchok JD (2018) Cancer immunotherapy using checkpoint blockade

A standard evaluation includes IGF-1, a complete metabolic panel, thyroid function, fasting glucose, and a hormonal panel appropriate for the patients cycle status

To measure the CRT expressions, after treatment, the cells were washed with cold PBS, and further incubated with anti-CRT polyclonal antibody (diluted 1:100, anti-human antibody for G7, WL1, U87, and U251 cells and anti-mouse antibody for GL261 and G422 cells) at 4 C for 30 min, and stained with Alexa Fluor 647 labeled goat antirabbit IgG (H&L), a highly cross-adsorbed secondary antibody (1:200 dilution), for 30 min at room temperature

Morphological examination, quantitative analyses of antioxidant concentrations, oxidative stress markers, Ca 2+ ion concentrations, Ca ATPase pump activities and apoptosis-associated proteins in the lenses were evaluated