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Description

The coagulation cascade required for serum preparation triggers massive in vitro platelet activation, artificially releasing abundant platelet-derived exosomes that severely skew and mask the true in vivo circulating exosomal profile (140, 141)

To determine cell viability, values were interpolated such that the control group (no VACV infection) was equivalent to 100% cell viability, whereas the cell death control (Triton 10%) was equivalent to 0%