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Last reviewed: June 1, 2026 Last updated: June 1, 2026 Written by: Jay Hastings, CEO of PlexusDx Jay Hastings is the CEO of PlexusDx, a precision health company focused on genetic testing, blood biomarker insights, and personalized wellness recommendations

Affinity chromatography is an important tool for selective biochemical separations This method uses a biologically-related binding agent as the stationary phase A literature survey is made of trends in this field over the past 50 years The principles, history, and applications of affinity chromatography are discussed Supports, coupling methods, and binding agents for this method are summarized Acknowledgements This work was funded, in part, by the University of Nebraska Research Council, the University of Nebraska-Lincoln College of Arts and Sciences, and the National Institutes of Health under grant R01 DK069629