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Radiolabeled proline (L-[ 3 H]Pro), serine (L-[ 3 H]Ser), glutamine (L-[ 3 H]Gln), asparagine (L-[ 3 H]Asn), leucine (L-[ 3 H]Leu), and glutamate (L-[ 3 H]Glu) (Perkin Elmer) (3 Ci/mL) were used to assess mitochondrial amino acid uptake by the addition of labeled amino acids to the respective tubes, followed by incubation at 37 C for one h

In contrast, in the presence of SOR the activation of thiamine biosynthetic genes was found not to be markedly different from wild type plants, suggesting that some additional, e.g