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Then, the samples were vigorously vortexed, incubated at RT for 20 min and centrifuged (4 C, 12,000 g , 20 min)

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HES-derived hCM were cultured on Matrigel TM -coated plates in RPMI 1640 supplemented with GlutaMAX, 1 mmol/L sodium pyruvate, 100 U/ml penicillin, 100 g/ml streptomycin (all Thermo Scientific), 200 mol/L L-ascorbic acid-2-phosphate sesquimagnesium salt hydrate (Sigma-Aldrich) and 2% B27 supplement (Thermo Scientific)