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Rats were survived for 5 additional days to allow for maximal retrograde transport of the tracer FG to the perikaya of spinal motor neurons, and sacrificed under deep anesthesia by transcardial perfusion with saline and subsequently buffered 4% paraformaldehyde

Liposomes were also made by mixing PC (Sigma-Aldrich, P3841), PE (Sigma-Aldrich, P7693), PS (Sigma-Aldrich P5660) and PIP 3 (P-3916, Echelon Biosciences)

230 Effective neuromuscular control relies on the precise regulation of ion channels and neurotransmitter release at the neuromuscular junction, which are modulated by molecular mechanisms