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Cells were deprived of growth factors by incubation in starvation media RPMI-1640 containing 0.5% (w/v) BSA (Sigma, St Louis, MO, USA) for 18 h before stimulation experiment with HGF (40 ng ml 1 for 7.5 min)

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An increase in the activity of matrix-metalloproteases (MMPs) or an increase in neuronal glucose uptake are two likely mechanisms by which inhibition of IRAP can provide an enhancement of memory

This mechanism promotes early satiety signals and reduces the rate of nutrient absorption, leading to flattened postprandial glucose excursions and reduced caloric intake