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Cells were washed three times with PBS and incubated with a donkey anti-mouse Alexa Fluor 568 (ThermoFisher Scientific, A10037) and donkey anti-rabbit Alexa Fluor 488 (ThermoFisher Scientific, A21206) secondary antibodies (1:400) for one hour in the dark

To summarize existing candidates for clinical radioprotectors, we conducted a literature review using a Pubmed/MEDLINE search with key phrases including: antioxidants in medical imaging, antioxidants in radiotherapy, antioxidant radiation, radioprotective agents, radioprotective radiotherapy, radioprotective medical imaging, and radioprotection. To be included, the articles were required to be peer-reviewed primary research articles published in the past 10 years that investigated one or more substances as potential radioprotective agents

Finasteride inhibits mainly 5AR2, while dutasteride blocks both Type I and Type II

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