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Materials and methods Strains, Plasmids and growth conditions The strains used in this study are listed in Table 1 and plasmids in Table 2

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PLs containing PUFAs are the primary substrates for lipid peroxidation in ferroptosis and are upregulated by enzymes such as acyl-CoA synthetase long-chain family member 4 (ACSL4), lysophosphatidylcholine acyltransferase 3 (LPCAT3), ALOXs, and Cytochrome P450 oxidoreductase(POR) (41).The membrane remodeling enzymes, ACSL4 (42) and LPCAT3, are key drivers of ferroptosis

A study published in *The Journal of Clinical Investigation* demonstrated that Glutathione deficiency was associated with oxidative stress and liver damage, and that IV Glutathione infusions could restore antioxidant levels and protect against liver injury