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The extraction was done in a buffer [1.0 mm ethylenediaminetetraacetic acid (EDTA), 100 mM TrisHCl (pH 7.3), 100 mM -mercaptoethanol, 1.0 mM phenyl methyl sulfonyl fluoride, 0.1 mM protease inhibitor cocktail, 2% glycerol, 25 mM MgCl 2 , 10 mM dipotassium hydrogen phosphate (K 2 HPO 4 )] and centrifuged at 12,000 g for 15 min, 4 C

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Emodin significantly mitigates cyclophosphamide-induced testicular toxicity in rats by improving sperm function and reproductive hormones levels, reversing oxidative stress and inflammation, and increasing testicular antioxidant enzymes, demonstrating its potential to counteract chemotherapy-related testicular damage [492]