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Studies indicate that exposure to PS-NPs significantly downregulates dopamine levels in zebrafish (Ren et al., 2024), mechanistically linked to MPs-triggered neuroinflammation that inhibits tyrosine hydroxylase, a key enzyme in dopamine synthesis (Wang et al., 2025)

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Changes in gut microbiota have been linked to several neurological conditions such as autism, anxiety, depression, schizophrenia, migraine, and neurodegeneration (Mohajeri et al., 2018

Subsequently, the cells were incubated with primary antibody (1:200) at 4 C for 15 h, followed by five washes with PBS