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Following a 3 min wash with 0.3% formic acid at a flow rate of 300 l min 1 , proteins were eluted with a solution of 40% isopropanol, 5% ACN and 0.3% formic acid at a flow rate of 40 l min 1

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Over the last two decades there has been studies that has changed in the view of the role of the luminal microbiota to also include significant impacts on host, via interactions with the GIT that may even be bidirectional

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