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To achieve early diagnosis and overall management of PD, key techniques and challenges include constructing a clinical database, bolstering PD early biomarker research, interpreting the clinical significance of biomarkers, enhancing interdisciplinary collaboration, establishing a joint diagnosis and AI diagnostic system using multiple biomarkers, as well as setting up a one-stop diagnostic and treatment center in partnership with community-based health service centers to screen, manage, and educate the high-risk PD populations

For instance, the probiotic strain Lactococcus lactis MG1363-pMG36e-GLP-1 activates the Nrf2/GPX4 signaling pathway to inhibit ferroptosis, ameliorating motor deficits in PD mice (Yue et al., 2022)

Quantitative metabolite profiling typically comes after qualitative profiling because the understanding of how much is often as important as what is it, especially as the drug makes its way along the discovery and development path

In this chapter, we explain the widely used GST pull-down assay to identify the proteinprotein interaction between purified proteins