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But what is unexpected is that although the effect of each reagent in the small test stage is not significantly different, but in the pilot test stage (that is, the experimental scale-up), we found that compared with other reagent combinations, the purity of the crude product reacted with TBTU/HOBt is better, and the small The purity of the crude product in the pilot test can reach more than 78%, and the purity of the crude product in the pilot scale production can also reach 75% under the condition of basically not reducing the yield