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Allergic Responses: Rarely, some people may develop mild allergic reactions, such as a skin rash or irritation

When comparing transcriptional features attributed to CPR [74] with the transcriptional response to allyl-ITC, a large overlap was detected within the induced response: of the 148 upregulated genes of the CPR, 111 genes were induced by allyl-ITC after 1 h and 132 genes were induced by allyl-ITC after 9 h (Additional file 4A and B)

In parallel with MTXs most well-known targets, several NAD+-dependent enzymes were inhibited by the antifolate in vitro : 2-oxoglutarate, isocitrate, malate, pyruvate, succinate, 6-phosphogluconate and glucose-6-phosphate dehydrogenases, glutamate-cysteine ligase, glutathione reductase, and glutathione peroxidase (29)

Studies have shown that the expression of TLR2, TLR4, and their co-receptor cluster of differentiation 14 (CD14) is significantly upregulated in the intestinal mucosa of IBD patients, particularly in active UC and CD cases [8].This upregulation may lead to an excessive recognition of gut microbiota, triggering abnormal inflammatory responses