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As a proof-of-principle, targeted amplicon sequencing across TP53 gene on plasma DNA samples collected from a limited number of localised prostate cancer patients before and after local treatment suggests detection of ctDNA before surgery is associated with poor clinical outcome (metastatic-free survival) [108]

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MI, by mitigating IR, decreases insulin-driven thecal cell steroidogenesis and hepatic inhibition of sex hormone-binding globulin (SHBG), effectively reducing free androgen bioavailability (228)