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mouse anti--actin, dilution 1:1000) were added for overnight incubation at 4 C and the antibody-bound proteins were developed with the enhanced chemiluminescence reagent coupled with horseradish peroxidase-conjugated secondary antibody

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Genetic lineage tracing has shown that 84% of newly formed cells originate from CYP2E1 + cells located around necrotic areas in a CCl 4 -induced liver injury model