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To precipitate the insoluble fraction, the solutions were centrifuged at 16,000 g for 10 min at 4 C

For quantification, Quantity One 29.0 and ImageLab 6.0.1 softwares (Bio-Rad, USA) were used, and the antibodies that were used are detailed in Supplementary Table 7

Moreover, Con A-induced PLC and I B phosphorylation was decreased by pretreatment of MS (0.4 mM, Gpx inhibitor) for 48 h in Jurkat cells, whereas those phosphorylation did not decrease by NAC (5 mM) in Jrukat cells with pretreatment of MS (0.4 mM, Gpx inhibitor) (Figure 7d)

Subsequently, the neurons undergo the classical reaction of ferroptosis (the Fenton reaction) (56)