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Consistent with its disruptions of mitochondrial respiration reflected in the cells OCR measurements, CB-839, starting at low concentrations of 0.1 to 1 M, also decreased the ATP pool in the HG-3 (P = 0.02) and MEC-1 (P = 0.01) cell lines, as measured using high-performance liquid chromatography ( Figure 2D ).The levels of other nucleotides remained stable at all concentration points

U.KwonE.SorhagenK.et al (2008)

aureus persister frequencies, RAW 264.7 macrophages or BMDMs were seeded into 24-well tissue culture-treated plates (Corning) at 4 10 5 cells per well in 500 l complete MEM, and incubated at 37 C and 5% CO 2 for 16 h

This blocks the combining of cysteine and glutamate before being joined with glycine to form more glutathione